RbohB, a Phaseolus vulgaris NADPH oxidase gene, enhances symbiosome number, bacteroid size, and nitrogen fixation in nodules and impairs
mycorrhizal colonization
reactive oxygen species (ros)
期刊名称： New Phytologist
年份： 2014年
期号： 第3期
关键词： arbuscular mycorrhizal fungi (AMF);bacteroids;infection threads;NADPH oxidase homologs;nodule senescence;Phaseolus vulgaris symbiosis;reactive
oxygen species (ROS);symbiosomes
摘要：The reactive oxygen species (ROS) generated by respiratory burst oxidative homologs (Rbohs) are involved in numerous plant cell signaling processes, and have critical roles in the symbiosis between
legumes and nitrogen-fixing bacteria. Previously, down-regulation of RbohB in Phaseolus vulgaris was shown to suppress ROS production and abolish Rhizobium infection thread (IT) progression, but also to enhance arbuscular mycorrhizal fungal (AMF) colonization. Thus, Rbohs function both as positive and negative regulators. Here, we assessed the effect of enhancing ROS concentrations, by overexpressing PvRbohB, on the P. vulgarisrhizobia and P. vulgarisAMF symbioses.We estimated superoxide concentrations in hairy roots overexpressing PvRbohB, determined the status of early and late events of both Rhizobium and AMF
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