AATCC 100 纺织品材料上耐细菌整理：评定Antibacterial Finishes on Textile Materials
Copyright ?? 2005 American Association of Textile Chemists and Colorists
AATCC Technical Manual /2006 TM 100-2004 149
Developed in 1961 by AATCC Committee
RA31; revised 1965, 1981, 1988 (with ti-
tle change), 1993, 1999; editorially re-
vised 1969, 1971, 1974, 1985;
reaffirmed 1977, 1981, 1989, 1998; edi-
torially revised and reaffirmed 1986,
2004.
1. Purpose and Scope
1.1 This test method provides a quanti-
tative procedure for the evaluation of the
degree of antibacterial activity. Assess-
ment of antibacterial finishes on textile
materials is determined by the degree of
antibacterial activity intended in the use
of such materials. If only bacteriostatic activity (inhibition of multiplication) is intended, a qualitative procedure which clearly demonstrates antibacterial activity as contrasted with lack of such activity by an untreated specimen may 9><>be accept- able. However, if bactericidal activity is intended or implied, quantitative evalua- tion is necessary. Quantitative evaluation also provides a clearer picture for possi- ble uses of such treated textile materials.
2. Principle
2.1 Swatches of test and control textile materials are tested qualitatively for anti- bacterial activity by AATCC Method 147. Those showing activity are evaluated quantitatively. Test and control swatches are inoculated with the test organisms.
After incubation, the bacteria are eluted from the swatches by shaking in known amounts of neutralizing solution. The number of bacteria present in this liquid is determined, and the percentage reduction
by the treated specimen is calculated.
3. Terminology
3.1 activity, n.—of an antibacterial
agent, a measure of effectiveness of the agent.
3.2 antibacterial agent, n.—in tex-
tiles, any chemical which kills bacteria (bactericide) or interferes with the multi- plication, growth or activity of bacteria (bacteriostat).
4. Safety Precautions
NOTE: These safety precautions are
editoriallyfor information purposes only. The pre- cautions are ancillary to the testing proce- dures and are not intended to <>be all inclu- sive. It is the user’s responsibility to use safe and proper techniques in handling materials in this test method. Manufac-
turers MUST <>be consulted for specific
details such as material safety data sheets and other manufacturer’s recommenda-
tions. All OSHA standards and rules
must also <>be consulted and followed.
4.1 Both the qualitative and quantita-
tive tests should <>be carried out by persons with training and experience in the use of bacteriological techniques. The U.S. De- partment of Health and Human Services publication, Biosafety in Microbiological
and Biomedical Laboratories, should <>be consulted (see 13.1).
4.2 CAUTION: Some of the bacteria
used in this test are capable of infecting humans and producing disease. There-
fore, every necessary and reasonable pre- caution must <>be taken to eliminate this
risk to the laboratory personnel and to personnel in the associated environment. Wear protective clothing and respiratory protection that prevents penetration by
the bacteria.
4.3 Good laboratory practices should
<>be followed. Wear safety glasses in all laboratory areas.
4.4 All chemicals should <>be handled
with care.
4.5 An eyewash/safety shower should
<>be located nearby for emergency use.
4.6 Sterilize all contaminated samples
and test materials prior to disposal.
4.7 Exposure to chemicals used in this procedure must <>be controlled at or below levels set by government authorities (e.g., Occupational Safety and Health Adminis- tration’s [OSHA] permissible exposure
limits [PEL] as found in 29 CFR
1910.1000 of January 1, 1989). In addition, the American Conference of Governmen-
tal Industrial Hygienists (ACGIH) Thresh- old Limit Values (TLVs) comprised of time weighted averages (TLV-TWA), short term exposure limits (TLV-STEL) and ceiling